Type 1 and type 2 responses to Leishmania major.

Evaluation of immune response against autoclaved Leishmania major incorporated into positively charged liposomes in Balb/c mice

Background and aim: Efficacy of vaccines is mainly depending on the type of adjuvant used. Efficacy trials of first generation vaccine against leishmaniasis showed a limited efficacy due to lack of an appropriate adjuvant. The objective of this study was to investigate whether positively charged liposomes containing ALM could induce Th1 type immune response in susceptible Balb/c mice.Mate...

The role of surface charge of ISCOMATRIX nanoparticles on the type of immune response generated against Leishmaniasis in BALB/c mice

Objective(s): ISCOMATRIX vaccines have now been shown to induce strong antigen-specific cellular or humoral immune responses to a broad range of antigens of viral, bacterial, parasite or tumor. In the present study, we investigated the role of ISCOMATRIX charge in induction of a Th1 type of immune response and protection against Leishmania major infection in BALB/c mice.  Materials and Methods:...

Bivalent DNA Vaccination with Genes Encoding Leishmania major Cysteine Proteinases Type I and II Protects Mice Against Infectious Challenge

Western Blot Analysis of Sera from Leishmania major-Infected BALB/c and C57BL/6 Mice

In Leishmania (L.) major-infected BALB/c mice Th2-type cells results in disease progression, whereas C57BL/6-infected mice mount a Th1-type response, which leads to control of the infection. Th2 response correlates with IgG1 whereas, Th1 response supports switching to IgG2a subclasses. Since IgG isotype-dominated response depends on different CD4 + T cell subsets, we studied the antigenic profi...

Adjuvant Effect of Leishmania major Promastigotes on the Immune Response of Mice to Ovalbumin

The immune responses of mice immunized with ovalbumin (OVA) together with killed L. major (KLM) promastigotes as adjuvant were studied. Three doses (5 × 107, 1 × 108 and 2 × 108) of KLM combined with OVA (100 mg) were injected into the groups of C57BL/6 mice. BCG and complete Freund’s adjuvant (CFA) were used as control adjuvants. Lymphocyte proliferation and antibody titers were determined, an...

Study on immunity of Leishmania major new crude antigen as a vaccine against leishmaniasis in out bred (resistant) and Balb/c (sensitive) mice

The aim of this study was to prepare a new formula of Leishmania major (L. major) crude antigen and evaluate its effect on immune system. For this purpose L. major promastigotes were cultured, harvested, washed, and resuspended in physiologic saline and the suspensions were dispersed in five equal batches. 0.1 ml of various doses of the cocktail antigen were injected intradermaly in three group...